Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/138444
Title: SINGLE CELL PAIRING MICROFLUIDIC APPLICATION FOR STUDYING IMMUNE MEDIATED KILLING WHEN PAIRED WITH TUMOUR CELLS
Authors: ONG XIAN DA LUCAS
Keywords: Single-Cell, Pairing, Microfluidic, Immune-Mediated, Killing, Tumour
Issue Date: 14-Aug-2017
Citation: ONG XIAN DA LUCAS (2017-08-14). SINGLE CELL PAIRING MICROFLUIDIC APPLICATION FOR STUDYING IMMUNE MEDIATED KILLING WHEN PAIRED WITH TUMOUR CELLS. ScholarBank@NUS Repository.
Abstract: Interactions between tumour and immune cells have been noted to elicit a huge variation of responses among patients undergoing cancer immunotherapy. However, current understanding of tumour to cancer interaction is still limited. I incorporated microfluidics to look at tumour-immune cell interaction at the single cell level and ultimately quantify immune cell-mediated killing. I trapped head and neck tumour cells from various patient-derived cell lines with varying numbers of natural killer cells and established a live-cell quantitative imaging assay to track the viability of the tumour cells longitudinally after a 24-hour co-culture. I then performed various statistical tests to identify differential responses between the cell types on-chip and between platforms. Differences in cell viability between cell types on-chip and platforms were observed. I believe that this platform will be a useful tool in allowing researchers to look objectively at tumour-immune cell interactions and will play a role in personalized cancer immunotherapy.
URI: http://scholarbank.nus.edu.sg/handle/10635/138444
Appears in Collections:Master's Theses (Open)

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