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|Title:||SINGLE CELL PAIRING MICROFLUIDIC APPLICATION FOR STUDYING IMMUNE MEDIATED KILLING WHEN PAIRED WITH TUMOUR CELLS||Authors:||ONG XIAN DA LUCAS||Keywords:||Single-Cell, Pairing, Microfluidic, Immune-Mediated, Killing, Tumour||Issue Date:||14-Aug-2017||Citation:||ONG XIAN DA LUCAS (2017-08-14). SINGLE CELL PAIRING MICROFLUIDIC APPLICATION FOR STUDYING IMMUNE MEDIATED KILLING WHEN PAIRED WITH TUMOUR CELLS. ScholarBank@NUS Repository.||Abstract:||Interactions between tumour and immune cells have been noted to elicit a huge variation of responses among patients undergoing cancer immunotherapy. However, current understanding of tumour to cancer interaction is still limited. I incorporated microfluidics to look at tumour-immune cell interaction at the single cell level and ultimately quantify immune cell-mediated killing. I trapped head and neck tumour cells from various patient-derived cell lines with varying numbers of natural killer cells and established a live-cell quantitative imaging assay to track the viability of the tumour cells longitudinally after a 24-hour co-culture. I then performed various statistical tests to identify differential responses between the cell types on-chip and between platforms. Differences in cell viability between cell types on-chip and platforms were observed. I believe that this platform will be a useful tool in allowing researchers to look objectively at tumour-immune cell interactions and will play a role in personalized cancer immunotherapy.||URI:||http://scholarbank.nus.edu.sg/handle/10635/138444|
|Appears in Collections:||Master's Theses (Open)|
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