Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/138406
Title: UNDERSTANDING THE NEUROVIRULENCE OF ENTEROVIRUS 71
Authors: YEO HUIMIN
Keywords: EV71, neurovirulence, mouse model, NSC-34, NITD008, AG129
Issue Date: 4-Aug-2017
Citation: YEO HUIMIN (2017-08-04). UNDERSTANDING THE NEUROVIRULENCE OF ENTEROVIRUS 71. ScholarBank@NUS Repository.
Abstract: NITD008, an adenosine nucleoside analog, was shown to fully protect EV71-infected two-week old AG129 mice from morbidity and mortality. NSC-34, a murine motor neuron cell line, was found to be susceptible and permissive to EV71 infection, with S41 strain being the most infectious in NSC-34 cells while MS and C2 strains were impaired at entry, replication and/or egress. EV71 exited NSC-34 cells via autophagic vacuoles. S41-infected AG129 mice exhibited morbidity and mortality while MS- or C2-infected mice were asymptomatic. S41-infected mice had high virus titers and histopathological damage in the CNS and this was not detected in MS-/C2-infected mice. Substitution of the 149th amino acid in VP2 protein from isoleucine to lysine (VP2I149K) in S41 resulted in complete loss of infectivity in NSC-34 cells due to an impairment at entry. Infection of mice with VP2I149K ameliorated morbidity and mortality. Reduced viral titers in the spinal cord and brain were observed.
URI: http://scholarbank.nus.edu.sg/handle/10635/138406
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
YeoHM.pdf30.24 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

101
checked on Sep 18, 2020

Download(s)

6
checked on Sep 18, 2020

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.