Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/138022
Title: DYNAMICS OF DENGUE VIRUSS UNCOATING AND ANTIBODY BINDING
Authors: LIM XIN XIANG
ORCID iD:   orcid.org/0000-0003-2002-1514
Keywords: Dengue, Dynamics, Antibodies, Mass Spectrometry, HDXMS, Virus thermodynamics
Issue Date: 3-Aug-2017
Citation: LIM XIN XIANG (2017-08-03). DYNAMICS OF DENGUE VIRUSS UNCOATING AND ANTIBODY BINDING. ScholarBank@NUS Repository.
Abstract: Viral particle uncoating is a pre-requisite to viral infectivity and is under strict thermodynamic control. Dengue fever, caused by the dengue virus (DENV), is the most prevalent arthropod-borne disease. I have mapped the thermodynamic profiles of two different serotypes of DENV using hydrogen/deuterium exchange mass spectrometry (HDXMS), and these reveal serotype-specific profiles. Consequently, the two DENV serotypes exhibited differential serotype-specific expansion under temperature perturbation conditions that mimic vector to host transmission. Changes in epitope dynamics are an important consequence of the serotype-specific expansion. Therefore, I have mapped the epitopes and paratopes of DENV2 in complex two different antibodies, a serotype-specific and a broadly neutralizing antibody, under host temperature perturbation conditions. These have allowed the uncovering of the mechanisms of antibody action associated with DENV neutralization. These studies suggest that therapeutic interventions that are able to steer viruses away from their intrinsic thermodynamic-driven programs, hold tremendous potential for blocking viral infections.
URI: http://scholarbank.nus.edu.sg/handle/10635/138022
Appears in Collections:Ph.D Theses (Open)

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