Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/137861
Title: CORRELATION BETWEEN BREAST CANCER CELL DEFORMABILITY AND THEIR METASTATIC POTENTIAL
Authors: YASAMAN NEMATBAKHSH
Keywords: Cancer, Mechanics, Circulating Tumor Cells, Microfluidics, Atomic Force Microscopy, Breast Cancer, Epithelial to Mesenchymal Transition
Issue Date: 4-Aug-2017
Citation: YASAMAN NEMATBAKHSH (2017-08-04). CORRELATION BETWEEN BREAST CANCER CELL DEFORMABILITY AND THEIR METASTATIC POTENTIAL. ScholarBank@NUS Repository.
Abstract: Metastasis accounts for most cancer-related deaths. Epithelial to mesenchymal transition (EMT) has been known as a main mechanism that drives a cell to metastasize. Changes in mechanical properties during this process have shown a great potential in marker-free phenotyping of cancer cells. Our objective was to investigate the deformability of breast cancer cells as they become more metastatic. Our study focused on three cell lines of breast epithelial cells (MCF-10A), non-invasive breast cancer cells (MCF-7) and invasive breast cancer cells (MDA-MB-231) considering an increase in the metastatic potential from former to latter. We first used Atomic Force Microscopy to study viscoelasticity of these cells in adherent and suspended states. We then developed our custom-designed microfluidics as a higher throughput system to compare deformability of these cells as metastatic potential went up. Finally, we studied Circulating tumor cells and investigated how deformability of these cells compared to their degree of EMT.
URI: http://scholarbank.nus.edu.sg/handle/10635/137861
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
NematbakhshY.pdf20.89 MBAdobe PDF

OPEN

NoneView/Download

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.