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|Title:||CORRELATION BETWEEN BREAST CANCER CELL DEFORMABILITY AND THEIR METASTATIC POTENTIAL||Authors:||YASAMAN NEMATBAKHSH||Keywords:||Cancer, Mechanics, Circulating Tumor Cells, Microfluidics, Atomic Force Microscopy, Breast Cancer, Epithelial to Mesenchymal Transition||Issue Date:||4-Aug-2017||Citation:||YASAMAN NEMATBAKHSH (2017-08-04). CORRELATION BETWEEN BREAST CANCER CELL DEFORMABILITY AND THEIR METASTATIC POTENTIAL. ScholarBank@NUS Repository.||Abstract:||Metastasis accounts for most cancer-related deaths. Epithelial to mesenchymal transition (EMT) has been known as a main mechanism that drives a cell to metastasize. Changes in mechanical properties during this process have shown a great potential in marker-free phenotyping of cancer cells. Our objective was to investigate the deformability of breast cancer cells as they become more metastatic. Our study focused on three cell lines of breast epithelial cells (MCF-10A), non-invasive breast cancer cells (MCF-7) and invasive breast cancer cells (MDA-MB-231) considering an increase in the metastatic potential from former to latter. We first used Atomic Force Microscopy to study viscoelasticity of these cells in adherent and suspended states. We then developed our custom-designed microfluidics as a higher throughput system to compare deformability of these cells as metastatic potential went up. Finally, we studied Circulating tumor cells and investigated how deformability of these cells compared to their degree of EMT.||URI:||http://scholarbank.nus.edu.sg/handle/10635/137861|
|Appears in Collections:||Ph.D Theses (Open)|
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