Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/136942
Title: GENOME-WIDE SCREEN FOR REGULATORS FOR TERT TRANSCRIPTION
Authors: MUHAMMAD KHAIRUL RAMLEE
Keywords: Telomerase, CRISPR screen, genome editing, high throughput
Issue Date: 27-Jul-2017
Citation: MUHAMMAD KHAIRUL RAMLEE (2017-07-27). GENOME-WIDE SCREEN FOR REGULATORS FOR TERT TRANSCRIPTION. ScholarBank@NUS Repository.
Abstract: Telomeres are nucleoprotein structures found at the ends of chromosomes. In somatic cells, telomeres shorten as these cells divide. When telomere reaches a critically short length, these cells undergo replicative senescence and eventually die. In contrast, stem and progenitor cells have the ability to maintain their telomere length via the expression of an enzymatic complex called telomerase. Similarly, more than 85% of cancer cells are found to upregulate the expression of telomerase, conferring them with the potential to proliferate indefinitely. Telomerase reverse transcriptase (TERT), the catalytic subunit of the telomerase holoenzyme, is considered to be the rate-limiting factor in reconstituting telomerase activity in vivo. To date, the expression and function of human TERT is known to be regulated at various molecular levels (including genetic, mRNA, protein and subcellular localization) by a number of diverse factors. However, the mechanism by which TERT expression is upregulated in cancer, downregulated in differentiating cells, and maintained in stem and progenitor cells is still unclear. In order to elucidate this mechanism, we performed a genome-wide knock-out screen to look for regulators of TERT transcription using GFP as a molecular expression marker of TERT and we discovered novel modulators of TERT expression such as epigenetic modifiers.
URI: http://scholarbank.nus.edu.sg/handle/10635/136942
Appears in Collections:Ph.D Theses (Open)

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