Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/136921
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dc.titleSTRUCTURE DETERMINATION, BIOCHEMICAL CHARACTERIZATION, AND PROTEIN ENGINEERING OF THE ACYL CARRIER PROTEIN DOMAIN FROM 6-DEOXYERYTHRONOLIDE B SYNTHASE
dc.contributor.authorWANG ZILONG
dc.date.accessioned2017-10-12T18:00:14Z
dc.date.available2017-10-12T18:00:14Z
dc.date.issued2017-01-17
dc.identifier.citationWANG ZILONG (2017-01-17). STRUCTURE DETERMINATION, BIOCHEMICAL CHARACTERIZATION, AND PROTEIN ENGINEERING OF THE ACYL CARRIER PROTEIN DOMAIN FROM 6-DEOXYERYTHRONOLIDE B SYNTHASE. ScholarBank@NUS Repository.
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/136921
dc.description.abstractPolyketides are an important class of natural products. 6-Deoxyerythronolide B Synthase (DEBS) is a type 1 polyketide synthase that produces the Polyketide antibiotic drug erythromycin. DEBS is a large multi-domain enzyme complex containing multiple enzymatic domains, including acyl carrier protein (ACP). ACPs are involved in chain transfer and chain elongation during polyketide biosynthesis. Structural characterization of all seven ACPs could lead to a better understanding of the domain-domain interaction and the mechanism of action of the DEBS complex. Some polyketide synthases contains tandem ACP domains and the number of constituting ACPs may dictate the rate of product formation. We have engineered a similar tandem ACP architecture for the DEBS complex and have improved of the biosynthetic rate. Our study will provide structural and mechanistic information on single and tandem ACPs within the context of a modular type 1 polyketide synthase protein.
dc.language.isoen
dc.subjectpolyketide synthase, 6-Deoxyerythronolide B Synthase, acyl carrier protein, X-ray diffraction, tandem acyl carrier protein, biosynthesis
dc.typeThesis
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.contributor.supervisorYEW WEN SHAN
dc.contributor.supervisorJAYARAMAN SIVARAMAN
dc.description.degreePh.D
dc.description.degreeconferredDOCTOR OF PHILOSOPHY
dc.identifier.isiutNOT_IN_WOS
Appears in Collections:Ph.D Theses (Open)

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