Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/136861
Title: A NOVEL CRYOGENIC PREPARATION TECHNIQUE FOR NEAR-INSTANTANEOUS VITRIFICATION OF BIOLOGICAL SPECIMENS
Authors: GU JING
Keywords: vitrification,cryogenic,fixation
Issue Date: 26-Jan-2017
Citation: GU JING (2017-01-26). A NOVEL CRYOGENIC PREPARATION TECHNIQUE FOR NEAR-INSTANTANEOUS VITRIFICATION OF BIOLOGICAL SPECIMENS. ScholarBank@NUS Repository.
Abstract: Cryogenic preparation of biological specimen for charged particle microscopy gains advantages by its high resolution and faithful imaging results. However, the existing cryo-fixation techniques has intrinsic limitation to achieve large vitreous depth due to poor sample thermal conductivity. This thesis suggests a microwave-assisted cryogenic fixation technique for vitrification enhancement. An optimized jet freezing system integrated with microwave irradiation applicator is established for in-situ optical evaluation of vitrification. The experimental results indicate that microwave irradiation produces significant crystallization disruption up to 75 μm depth with optimized pulse train configurations. High microwave frequency is proven effective in achieving crystallization disruption, and pulsed signal with high pulse but low average power is predominant to eliminate undesired sample heating meanwhile maintain the crystallization disruption effect. This proposed technique suggests a novel cryogenic preparation technique to preserve the biological ultrastructures in atmosphere, which is also capable to be integrated with existing imaging facilities.
URI: http://scholarbank.nus.edu.sg/handle/10635/136861
Appears in Collections:Ph.D Theses (Open)

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