Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/13604
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dc.titleMolecular basis of Agrobacterium-mediated gene transfer into mammalian cells
dc.contributor.authorHOU QINGMING
dc.date.accessioned2010-04-08T10:34:40Z
dc.date.available2010-04-08T10:34:40Z
dc.date.issued2004-02-24
dc.identifier.citationHOU QINGMING (2004-02-24). Molecular basis of Agrobacterium-mediated gene transfer into mammalian cells. ScholarBank@NUS Repository.
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/13604
dc.description.abstractA novel system for detection of gene transfer between A. tumefaciens and mammalian cells was established. Using this system, Agrobacterium was found to be able to deliver DNA located on its plasmid and chromosome into human cells. Agrobacterium is actively involved in this process; such a kind of DNA transfer might occur widely between bacteria and mammalian cells. The frequency of such a gene transfer was approximately 10-4-10-5 transformants per recipient. Our data showed that this DNA transfer is dependent upon Agrobacterium and independent of its virulence genes. The polymerization of actin played an important role in Agrobacterium internalization and subsequent gene transfer into mammalian cells. In addition, an Agrobacterium sensor protein ChvG was found to be important for the gene transfer into both plant and mammalian cells. The data suggested that ChvG was involved in the regulation of acid-inducible genes and might function as a global sensor protein that can directly or indirectly sense extracellular acidity.
dc.language.isoen
dc.subjectAgrobacterium tumefaciens; mammalian cell; horizontal gene transfer (HGT); global pH sensor
dc.typeThesis
dc.contributor.departmentBIOLOGICAL SCIENCES
dc.contributor.supervisorPAN SHEN QUAN
dc.description.degreePh.D
dc.description.degreeconferredDOCTOR OF PHILOSOPHY
dc.identifier.isiutNOT_IN_WOS
Appears in Collections:Ph.D Theses (Open)

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