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|Title:||PROMOTING LIPID PRODUCTIVITY AND FATTY ACID SECRETION IN MICROALGAE||Authors:||TAN WEI MIN KENNETH||Keywords:||Microalgae, Dunaliella tertiolecta, Lipid productivity, Fatty acid secretion, Nitrogen depletion, Substrate provision||Issue Date:||4-Jan-2017||Citation:||TAN WEI MIN KENNETH (2017-01-04). PROMOTING LIPID PRODUCTIVITY AND FATTY ACID SECRETION IN MICROALGAE. ScholarBank@NUS Repository.||Abstract:||Microalgae biofuels suffer from high capital and operating costs due to low yields and costly extraction methods. Two main bottlenecks are: 1) finding the balance between increasing biomass productivity and lipid accumulation, and 2) reducing energy costs while extracting lipids from the cells. Here, we induced nitrogen depletion in the halotolerant microalga Dunaliella tertiolecta to identify enzymes which correspond with lipid accumulation. We found upregulation of several enzymes which catalyze the formation of acetyl-CoA and NADPH, substrates required for fatty acid (FA) synthesis. Overexpression of these enzymes in the model microalga Chlamydomonas reinhardtii resulted in up to 56% increase in lipid production without compromising growth. Next, we introduced ABCG11, a plant ATP-binding cassette transporter known to secrete FAs from plant cells, into C. reinhardtii and cyanobacteria. However, although we were able to increase FA production in the cells, we did not find evidence of FA secretion in the culture media.||URI:||http://scholarbank.nus.edu.sg/handle/10635/135849|
|Appears in Collections:||Ph.D Theses (Open)|
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