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|Title:||THE DEVELOPMENT OF SELECTIVE AND SENSITIVE SMALL-MOLECULE FLUORESCENT PROBES FOR IMAGING HOCL, ATP AND NADH IN LIVE CELLS||Authors:||WANG LU||Keywords:||fluorescence; probe; imaging; HOCl; ATP; NADH||Issue Date:||16-Dec-2016||Citation:||WANG LU (2016-12-16). THE DEVELOPMENT OF SELECTIVE AND SENSITIVE SMALL-MOLECULE FLUORESCENT PROBES FOR IMAGING HOCL, ATP AND NADH IN LIVE CELLS. ScholarBank@NUS Repository.||Abstract:||Fluorescent probes have emerged as an essential tool in the molecular recognition events in biological systems. Due to the complex intracellular environment, it is essential and urgent to reasonably design fluorescent probe with high sensitivity and selectivity. In this work, we aim to develop fluorescent probes for sensitively and selectively imaging biomolecules. In chapter 2, based on design-based approach, we designed and prepared targetable two-photon fluorescent probes, showing fast response, good sensitivity, and high selectivity to HOCl, which was used to detect sub-cellular HOCl in live cell and inflamed mouse model. In chapter 3-5, we developed a boronic acid based fluorescence library (~80 compounds) for imaging diol-containing complicated biomolecules. After screening, we obtained a switchable ATP probe for specifically monitoring mitochondrial ATP levels fluctuation (chapter 4). In chapter 5, a “turn-on” fluorescent probe, showing excellent sensitivity and selectivity to NADH, was prepared for imaging NADH levels in live cells. These work provide three excellent tools for sensitively and selectively imaging intracellular HOCl, ATP, and NADH, as well as novel strategies for developing future probes.||URI:||http://scholarbank.nus.edu.sg/handle/10635/135841|
|Appears in Collections:||Ph.D Theses (Open)|
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