Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/135780
Title: INVESTIGATING HOW ANTI-PRM ANTIBODIES TURN IMMATURE DENGUE VIRUS INFECTIOUS
Authors: MELISSA WIRAWAN
Keywords: maturation, dengue, infectivity, antibody, enhancement, cryoEM
Issue Date: 6-Feb-2017
Citation: MELISSA WIRAWAN (2017-02-06). INVESTIGATING HOW ANTI-PRM ANTIBODIES TURN IMMATURE DENGUE VIRUS INFECTIOUS. ScholarBank@NUS Repository.
Abstract: Dengue virus (DENV) is initially assembled as an immature particle with heterotrimeric spikes of envelope (E) and pre-membrane (prM) proteins. The large proportion of anti-prM antibodies in DENV-infected patients suggests presence of immature DENV particles. Fully immature DENV is non-infectious on its own but can turn infectious towards Fcγ-receptor-bearing cells when complexed with anti-prM antibody. After endocytosis, maturation involving dissociation of pr-peptides is crucial for infectivity. It is however, unknown how pr-peptides dissociate at low pH as the dissociation requires exposure to neutral pH. Here, using cryo-Electron Microscopy, we determined the structures of immature DENV-anti-prM Fab complex at pH 8.0 and 5.0, simulating environment before cell entry and after endocytosis. At pH 5.0, there were fewer Fabs on the virus and the E protein had been rearranged. Furthermore, presence of Fab enhanced virus particles-liposomes interaction. Taken together, our results suggest anti-prM antibodies assist in entry and interaction with endosomal membrane.
URI: http://scholarbank.nus.edu.sg/handle/10635/135780
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
WirawanM.pdf23.47 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

94
checked on Nov 24, 2022

Download(s)

14
checked on Nov 24, 2022

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.