Please use this identifier to cite or link to this item:
https://scholarbank.nus.edu.sg/handle/10635/135741
Title: | UNDERSTANDING OUTER MEMBRANE LIPID ASYMMETRY IN ESCHERICHIA COLI | Authors: | CHONG ZHI SOON | Keywords: | Gram-negative bacteria, outer membrane, lipid asymmetry, OmpC-Mla system, phospholipid transport, bile salts | Issue Date: | 21-Dec-2016 | Citation: | CHONG ZHI SOON (2016-12-21). UNDERSTANDING OUTER MEMBRANE LIPID ASYMMETRY IN ESCHERICHIA COLI. ScholarBank@NUS Repository. | Abstract: | Gram-negative bacteria are hard to treat because of their outer membranes (OMs), which function as a permeability barrier against many antibiotics and bile salts. Disruption to the asymmetry of the OM can lead to migration of phospholipids to the outer leaflet of the OM, which compromises the integrity of the OM. We demonstrate that in Escherichia coli, the Mla system maintains OM lipid asymmetry with the help of osmoporin OmpC. We show that OM lipoprotein MlaA interact specifically with OmpC and OmpF, and removing OmpC from cells causes phospholipid accumulation in the outer leaflet of the OM in stationary phase. We further show that cells lacking MlaA or its binding partner OmpC are sensitive to bile salts in a manner independent of OM outer leaflet phospholipid accumulation. We conclude that MlaA and OmpC may function together in maintaining OM lipid asymmetry and providing bile salt resistance in enteric Escherichia coli. | URI: | http://scholarbank.nus.edu.sg/handle/10635/135741 |
Appears in Collections: | Ph.D Theses (Open) |
Show full item record
Files in This Item:
File | Description | Size | Format | Access Settings | Version | |
---|---|---|---|---|---|---|
ChongZS_Understanding outer membrane lipid asymmetry in Escherichia coli.pdf | 7.07 MB | Adobe PDF | OPEN | None | View/Download |
Google ScholarTM
Check
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.