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|Title:||HETEROCHROMATIN PROTEIN 1A REGULATES TRANSPOSONS VIA A COMPARTMENTALIZED piRNA PATHWAY IN DROSOPHILA FEMALE GERMLINE||Authors:||TEO YEE WEI||Keywords:||Heterochromatin Protein 1a, Piwi-interacting RNAs, Transposons, Transposable elements, female germline, Drosophila melanogaster||Issue Date:||18-Jan-2017||Citation:||TEO YEE WEI (2017-01-18). HETEROCHROMATIN PROTEIN 1A REGULATES TRANSPOSONS VIA A COMPARTMENTALIZED piRNA PATHWAY IN DROSOPHILA FEMALE GERMLINE. ScholarBank@NUS Repository.||Abstract:||In metazoan germline, Piwi-interacting RNAs (piRNAs) provide defence against transposons. Piwi-piRNAs complex in nucleus, along with downstream components, mediates transcriptional silencing of transposons. Heterochromatin Protein 1a (HP1a) is proposed to function downstream to Piwi-piRNA complex for transposon silencing in Drosophila female germline. I show that HP1a represses subset of transposons, predominantly in telomeric and pericentric regions by maintaining overall and Piwi-bound piRNAs targeting them. HP1a is required to repress evolutionarily older transposons, which are enriched in pericentric regions. HP1a-mediated transposon repression is loci-dependent. Loss in piRNA targeting transposons in pericentric and telomeric regions upon HP1a germline knockdown is highly correlated with HP1a enrichment at these regions. In addition, HP1a is not required for the events upstream to piRNA processing. I show that HP1a functions to maintain piRNAs, downstream to processing, at the regions it is enriched. Thus, piRNA maintenance is likely compartmentalised by chromosomal locations potentially through HP1a genomic localisation.||URI:||http://scholarbank.nus.edu.sg/handle/10635/135643|
|Appears in Collections:||Ph.D Theses (Open)|
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