Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/135639
Title: ALTERING FATTY ACID PROFILE IN MICROALGAE FOR IMPROVED BIODIESEL PRODUCTION
Authors: LIN HUIXIN
Keywords: Microalgae, Fatty acid, Lipid, Genetic engineering, Transcriptomics, Thioesterase
Issue Date: 11-Jan-2017
Citation: LIN HUIXIN (2017-01-11). ALTERING FATTY ACID PROFILE IN MICROALGAE FOR IMPROVED BIODIESEL PRODUCTION. ScholarBank@NUS Repository.
Abstract: Metabolic engineering of microalgae to accumulate high levels of medium chain length fatty acids (MCFAs) represents an attractive strategy to improve the fuel performance properties of biodiesel, but it has met with limited success. Traditional approaches employ single introduction of MCFA specific acyl-ACP thioesterases (TEs), but our current research in transgenic Dunaliella tertiolecta lines highlights that there is no single rate limiting approach to redirect FA synthesis, from long to medium chain lengths. Here, we explore the accumulation of MCFAs in D. tertiolecta after transgenic expression of two MCFA specific TEs (C12TE, C14TE), respectively. All genetic modifications were performed using our optimized transformation system for D. tertiolecta to achieve stable transgenic lines. From various ‘omics’ analyses, our study observed the correlation between gene distribution and MCFA production, and revealed three main interacting pathways involved, namely, FA synthesis (C12TE, C14TE, KASIV, KASII, Δ9D, Δ12D), FA transport (LACS), and peroxisomal β-oxidation (ACX3).
URI: http://scholarbank.nus.edu.sg/handle/10635/135639
Appears in Collections:Ph.D Theses (Open)

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