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|Title:||Temperature sensitive poly (N-isopropylacrylamide) hydrogels for protein delivery||Authors:||WU JING YI||Keywords:||Temperature-responsive;Hydrogels;Protein Delivery;Protein-Gel Interactions;Lower Critical Solution Temperature;Semi-interpenetrating Polymer Networks||Issue Date:||17-Dec-2003||Citation:||WU JING YI (2003-12-17). Temperature sensitive poly (N-isopropylacrylamide) hydrogels for protein delivery. ScholarBank@NUS Repository.||Abstract:||Thermosensitive poly(N-isopropylacrylamide) (PNIPAAm) hydrogels were synthesized and investigated as protein delivery devices. In vitro protein release from PNIPAAm gels were studied under various conditions: different crosslinking contents, release temperatures, protein loading and molecular size as well as temperature cycling. There was a non-release phase observed due to protein-gel interactions, as proven by a shift in the lower critical solution temperature (LCST). Therefore, linear poly(vinyl alcohol) (PVA) chains were added to crosslinked PNIPAAm gels to form semi-interpenetrating networks (semi-IPNs) to improve protein release efficiency. The semi-IPNs showed higher swelling ratios and greater temperature sensitivities than PNIPAAm gels. Greater percentage of protein released and no shift in LCST of semi-IPN gels after release indicated that protein-gel interactions were hindered by PVA that occupied the limited binding sites within PNIPAAm network. Consequently, the protein-gel interactions were minimized and protein delivery efficiency from PNIPAAm gels was enhanced.||URI:||http://scholarbank.nus.edu.sg/handle/10635/13558|
|Appears in Collections:||Master's Theses (Open)|
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