Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/135504
Title: EFFICIENT GENERATION OF NEURAL CREST STEM CELLS AND NEURAL CREST LINEAGES FROM HUMAN EMBRYONIC STEM CELLS
Authors: ZHU QIAN
Keywords: Differentiation, Human embryonic stem cells, Keratocyte, Neural crest stem cells, Peripheral neuron
Issue Date: 16-Dec-2016
Citation: ZHU QIAN (2016-12-16). EFFICIENT GENERATION OF NEURAL CREST STEM CELLS AND NEURAL CREST LINEAGES FROM HUMAN EMBRYONIC STEM CELLS. ScholarBank@NUS Repository.
Abstract: Neural crest stem cells (NCSCs) as a multipotent cell population are capable of differentiating into diverse cell types. Since it is extremely challenging to isolate NCSCs from embryos, alternative unrestricted sources of NCSCs have to be explored. Here, a feeder-free system was established to efficiently generate NCSCs (P75+/HNK1+/ AP2α+/PAX6-) from hESCs in large quantities and high purity within 4 days. These hESC-derived NCSCs could maintain the self-renewal capacity in vitro until at least day-8. In addition, they were able to generate mesenchymal stem cells (CD73+CD90+CD105+), peripheral neurons (peripherin+ β-tubulin III +) with essential electrophysiological function, and corneal keratocytes (keratocan+). What is more, they also possessed the in vivo development potential. The feeder-free monolayer culture system for keratocyte differentiation is a significant finding in this project since it has never been reported before. Considering the importance of NCSCs, this work may provide a useful platform for relevant laboratory research and clinical applications.
URI: http://scholarbank.nus.edu.sg/handle/10635/135504
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