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|Title:||DIVERSE WAYS OF CONTROLLING miRNA EXPRESSION DURING FLY DEVELOPMENT||Authors:||ZHOU LI||Keywords:||miRNA, transcriptome, RNA-seq, regulation, mechanism||Issue Date:||16-Jan-2017||Citation:||ZHOU LI (2017-01-16). DIVERSE WAYS OF CONTROLLING miRNA EXPRESSION DURING FLY DEVELOPMENT. ScholarBank@NUS Repository.||Abstract:||As significant gene regulators, miRNAs are processed from long mRNA-like transcripts (primary miRNAs) by sequential actions of ribonucleases Drosha and Dicer. But how the expression of miRNAs could be controlled at each step is currently unknown. To explore the underlying mechanisms, we did expression profiling analyses on mature and primary miRNAs from small and long RNA-seq libraries, respectively, which were generated from a time course covering the whole Drosophila embryogenesis. Although majority miRNAs generated from the same polycistronic transcript, also called ‘clustered miRNAs’, tended to show similar expression patterns, dissimarility in expression changes between individual members of clusters was also observed, indicating that the transcription level was not the only determinant. Further analyses showed that distinctive miRNA stabilities, alternative promoter usage and differential Drosha cleavage efficiency were some of the bases of the differential regulation of miRNA clusters, demonstrating that miRNAs were regulated by diverse mechanisms.||URI:||http://scholarbank.nus.edu.sg/handle/10635/135501|
|Appears in Collections:||Ph.D Theses (Open)|
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