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|Title:||SYNTHETIC IMPORT SUBSTRATES REVEAL CROSSTALK BETWEEN PEROXISOME MATRIX AND MEMBRANE PROTEIN IMPORT MACHINERIE||Authors:||YANG JING||Keywords:||peroxisome homeostasis, peroxisome matrix import, peroxisome membrane import||Issue Date:||18-Aug-2016||Citation:||YANG JING (2016-08-18). SYNTHETIC IMPORT SUBSTRATES REVEAL CROSSTALK BETWEEN PEROXISOME MATRIX AND MEMBRANE PROTEIN IMPORT MACHINERIE. ScholarBank@NUS Repository.||Abstract:||Peroxisomes possess the remarkable capacity to import complex protein oligomers. This is believed to occur through transient pores formed by the substrate, import receptors and membrane-associated importomer proteins. However, the effects of import and constraints imposed by the size and composition of cargo remain unclear. Here, we construct a series of synthetic import substrates and employ microinjection and quantitative microscopy to assess their fate in mammalian tissue culture cells. We find that single stranded DNA, double stranded DNA, and polysaccharides can be “piggyback” imported when attached non-covalently to a peroxisome targeted protein. Cargo with a diameter of 12.5 nm is efficiently imported, however, a 30 nm oligomer is not. Remarkably, we find that a single round of matrix import results in peroxisome growth, which coordinated with peroxisomal membrane protein increase proportionally, and favors subsequent peroxisomal localization of tail-anchored proteins that can traffic to either peroxisomes or mitochondria. Together, these data reveal a promiscuous import machinery and coupling between matrix and membrane protein import pathways.||URI:||http://scholarbank.nus.edu.sg/handle/10635/134851|
|Appears in Collections:||Ph.D Theses (Open)|
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