Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/134814
Title: FREQUENCY AND AMPLITUDE CONTROL OF CORTICAL OSCILLATIONS BY LIPID METABOLISM
Authors: XIONG DING
Keywords: phsophoinositides, cortical oscillation, frequency, amplitude, ER dynamics
Issue Date: 18-Aug-2016
Citation: XIONG DING (2016-08-18). FREQUENCY AND AMPLITUDE CONTROL OF CORTICAL OSCILLATIONS BY LIPID METABOLISM. ScholarBank@NUS Repository.
Abstract: Dynamic patterns, such as oscillations and waves, of signaling factors are of great importance to cell physiology in single and multicellular systems. Currently, majority of the studies focus primarily on cytoskeleton-dependent feedbacks. In contrast, information on signaling mechanisms upstream of the actin cytoskeleton remains unclear. In my study, I report phosphoinositides traveling waves on the plasma membrane of rat basophilic leukemia (RBL) mast cells (Xiong et al. 2016). Sequential waves of phosphoinositides are generated by two phase-shifted phosphoinositide phosphatases namely synaptojanin 2 and SHIP1. By perturbing phosphoinositides using optogenetic methods and chemical drugs, I demonstrate that the pulses of PtdIns(4,5)P2 regulate the amplitude of cyclic membrane waves while PtdIns(3,4)P2 sets the frequency. Class I PI3Kδ is the main functional PI3K involved in regulating the frequency through phosphatase-dependent inhibitory action. Besides, dynamic patterns of global oscillations have been observed on the plasma membrane and the ER membrane coupling with calcium oscillations.
URI: http://scholarbank.nus.edu.sg/handle/10635/134814
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
Frequency and amplitude control of cortical oscillations by lipid metabolism.pdf21.6 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

80
checked on May 23, 2019

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.