Please use this identifier to cite or link to this item:
https://scholarbank.nus.edu.sg/handle/10635/134812
DC Field | Value | |
---|---|---|
dc.title | RECRUITMENT OF VINCULIN DURING TALIN STRETCHING | |
dc.contributor.author | HU XIAN | |
dc.date.accessioned | 2017-02-16T18:00:19Z | |
dc.date.available | 2017-02-16T18:00:19Z | |
dc.date.issued | 2016-07-29 | |
dc.identifier.citation | HU XIAN (2016-07-29). RECRUITMENT OF VINCULIN DURING TALIN STRETCHING. ScholarBank@NUS Repository. | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/134812 | |
dc.description.abstract | Talin is a critical focal adhesion adaptor protein that connects actin and integrin directly. Talin dimer has up to 22 cryptic vinculin binding sites (VBS) that are exposed by stretching. A method was developed to dynamically measure both the length of talin and the binding of vinculin at a superresolution level in live cells. Unlike many models of the talin dimer, talin N-termini(integrin-binding domain) are sperated by about 180nm, whereas the C-terminal dimerization domains colocalize. The rapid stretching and relaxation cycles of the talin dimers occurs majority in peripheral cell regions, with a direction that agrees with actin flow. This is consistent with a stick-slip model for transient binding to flowing actin. By tagging a vinculin-dihydrofaolate reductase(DHFR) chimera with a covalent fluorophore(TMP-atto655). Vinculin binding to talin was monitored in parallel with talin length. A peak of binding was found at the length of 180nm for both full-length vinculin and vinculin head domain, but controls didn’t bind. Surprisingly, multiple vinculins bound within a single second in narrowly localized regions of the talin rod during stretching. Thus we suggest that talin stretching activates vinculin binding in a cooperative manner, consistent with vinculin dynamics in vivo. | |
dc.language.iso | en | |
dc.subject | Mechanobiology, Protein Stretching, Superresolution, Talin, Vinculin, α-Actinin | |
dc.type | Thesis | |
dc.contributor.department | MECHANOBIOLOGY INSTITUTE | |
dc.contributor.supervisor | SHEETZ, MICHAEL | |
dc.contributor.supervisor | BERSHADSKY, ALEXANDER | |
dc.description.degree | Ph.D | |
dc.description.degreeconferred | PH.D. IN MECHANOBIOLOGY (NGS) | |
dc.identifier.isiut | NOT_IN_WOS | |
Appears in Collections: | Ph.D Theses (Open) |
Show simple item record
Files in This Item:
File | Description | Size | Format | Access Settings | Version | |
---|---|---|---|---|---|---|
SupplementaryMovie1.mp4 | 5.19 MB | MP4 | OPEN | None | Preview online | |
SupplementaryMovie2.mp4 | 15.73 MB | MP4 | OPEN | None | Preview online | |
SupplementaryMovie3.mp4 | 5.97 MB | MP4 | OPEN | None | Preview online | |
HUX.pdf | 26.08 MB | Adobe PDF | OPEN | None | View/Download |
Google ScholarTM
Check
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.