Please use this identifier to cite or link to this item:
Title: Mechanisms of GTP-depletion induced apoptosis in HIT-T15 insulin-secreting cells
Keywords: beta cell, mycophenolic acid, apoptosis, p21, caspase-2, tissue transglutaminase
Issue Date: 14-Oct-2003
Citation: HUO JIANXIN (2003-10-14). Mechanisms of GTP-depletion induced apoptosis in HIT-T15 insulin-secreting cells. ScholarBank@NUS Repository.
Abstract: Prolonged GTP depletion by mycophenolic acid (MPA) caused arrest of insulin-secreting beta-cells at G1 phase and triggered apoptosis in a dose- and time-dependent manner. Activation of caspase-2 was a critical event in this scenario, although it also involved activation of other caspases (caspase-3 and -9) and release of cytochrome c from mitochondria. In addition, the time course of MPA-induced apoptosis was closely correlated with the increase in tissue transglutaminase (tTG) activity. However, it appeared that tTG was only restricted to some terminal morphological changes but not responsible for the initiation of the cell death. Importantly, p21WAF1/CIP1 (a cyclin-dependent-kinase inhibitor) was elevated by MPA treatment independent of p53, an event preceding and well associated with caspase activation. A close relationship between p21WAF1/CIP1 and caspase activation/apoptosis was also observed when a p21WAF1/CIP1 inducer was studied. Thus these results indicate that p21WAF1/CIP1 may act as an upstream signal to activate caspases and in turn induce apoptosis subsequent of impaired mitogenesis due to sustained GTP-depletion.
Appears in Collections:Ph.D Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
HUOJX.pdf997.77 kBAdobe PDF



Page view(s)

checked on Mar 4, 2019


checked on Mar 4, 2019

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.