Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/134710
Title: ASSESSMENT OF HUMAN SPECIFIC MICROBIAL INDICATORS IN TROPICAL URBAN WATERS
Authors: LIANG LIANG
Keywords: microbial source tracking (MST) tools, Bacteroides thetaiotaomicron, Methanobrevibacter smithii, Human polyomaviruses, pathogens, persistence
Issue Date: 6-Oct-2016
Citation: LIANG LIANG (2016-10-06). ASSESSMENT OF HUMAN SPECIFIC MICROBIAL INDICATORS IN TROPICAL URBAN WATERS. ScholarBank@NUS Repository.
Abstract: The recent development of microbial source tracking (MST) markers, such as human polyomaviruses (HPyVs), Bacteroides thetaiotaomicron (B. theta) and Methanobrevibacter smithii (M. smithii), provides the potential for identifying sources of fecal contamination in surface waters. This study presents the validation of three MST markers, their occurrence and persistence, and their relationships with human pathogens in tropical surface waters. Among the three markers, HPyVs had the highest specificity (100%) to human fecal pollution, followed by M. smithii (97%) and B. theta (90%). Based on data collected in urban catchments of Singapore, the positive detection of B. theta showed the highest capability for identifying the safety level of recreational water quality. HPyVs has similar decay rates as human adenovirus in freshwater, while B theta may work better in seawater based on the survival models developed in this study. Hence, the combination of HPyVs and B. theta is useful for source tracking studies.
URI: http://scholarbank.nus.edu.sg/handle/10635/134710
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