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https://scholarbank.nus.edu.sg/handle/10635/134687
DC Field | Value | |
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dc.title | STUDY THE ROLE OF MICRO-NICHES IN EPITHELIAL APLICAL-BASAL POLARISATION | |
dc.contributor.author | WENG ZHE | |
dc.date.accessioned | 2017-01-31T18:00:47Z | |
dc.date.available | 2017-01-31T18:00:47Z | |
dc.date.issued | 2016-08-19 | |
dc.identifier.citation | WENG ZHE (2016-08-19). STUDY THE ROLE OF MICRO-NICHES IN EPITHELIAL APLICAL-BASAL POLARISATION. ScholarBank@NUS Repository. | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/134687 | |
dc.description.abstract | Epithelia apical-basal polarisation is important for the formation of a selective barrier. This thesis developed a novel micro-well based mini-organ approach to decouple the chemical and physical parameters, to control the 3-D micro-niches, and quantitatively assessed the localization of key polarity markers. This thesis work has reported that at single cell level, lateral input of cell-cell adhesion cue in a 3-D micro-niche is crucial for the formation of apically localized “zonula adherens” belt structure identified by an actin belt, together with PAR-3, N-WASP, ZO-1 and Claudin-3, but not supported by cell-matrix adhesion cues alone. 2-D input of cell-cell adhesion cues also induced an edge localized E-cadherin and actin belt. Taken together, this thesis demonstrates that cell-cell adhesion cues in 3-D and 2-D micro-niches are sufficient to develop “zonula adherens” like belt formation at single cell level, and creates a novel tool to quantitatively imaging and analysis of key polarity markers. | |
dc.language.iso | en | |
dc.subject | POLARITY, EPITHELIAL, MICRO-NICHE, ZONULA ADHERENS, MICRO-WELL, PATTERNING | |
dc.type | Thesis | |
dc.contributor.department | MECHANOBIOLOGY INSTITUTE | |
dc.contributor.supervisor | VIASNOFF, VIRGILE NICOLAS ROBE | |
dc.description.degree | Ph.D | |
dc.description.degreeconferred | PH.D. IN MECHANOBIOLOGY (NGS) | |
dc.identifier.isiut | NOT_IN_WOS | |
Appears in Collections: | Ph.D Theses (Open) |
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File | Description | Size | Format | Access Settings | Version | |
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WENG ZHE_PhD Thesis_A0089334X_Final.pdf | 36.59 MB | Adobe PDF | OPEN | None | View/Download |
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