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|Title:||A SYNTHETIC BIOLOGY APPROACH TO INVESTIGATE THE INTERACTIONS OF MOUSE CRIPTO||Authors:||SACHIN SESHADRI SREEPATHY||Keywords:||Cripto, p-benzoylphenylalanine, cross-linking, Nodal signaling, synthetic biology, protein-protein interactions||Issue Date:||16-Aug-2016||Citation:||SACHIN SESHADRI SREEPATHY (2016-08-16). A SYNTHETIC BIOLOGY APPROACH TO INVESTIGATE THE INTERACTIONS OF MOUSE CRIPTO. ScholarBank@NUS Repository.||Abstract:||The Nodal signaling cascade is a signaling pathway that is conserved in vertebrates and plays a central role in mesendoderm specification. The interactions of the Nodal signaling co-receptor, Cripto, are unclear in the existing literature. We addressed this problem by using a synthetic biology approach that utilizes the site-specifically installed cross-linking amino acid, p-benzoylphenylalanine, to tease out direct interactions in live HEK293 cells. We identified a fundamental flaw in the Cripto literature and determine that using a C-terminally tagged version does not impart the correct membrane localization. We created a library of N-terminally tagged, glycosylated mutants of Cripto containing p-benzoylphenylalanine at specific sites that can pave the way for understanding its direct, physical interactions.||URI:||http://scholarbank.nus.edu.sg/handle/10635/134683|
|Appears in Collections:||Master's Theses (Open)|
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