Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/134549
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dc.titlePHOSPHORYLATION OF TROPOMODULIN3 BY AMPK REGULATES GLUT4 TRANSLOCATION AND GLUCOSE UPTAKE IN L6 MYOBLASTS
dc.contributor.authorMAN MOHAN SHRESTHA
dc.date.accessioned2017-01-12T18:00:13Z
dc.date.available2017-01-12T18:00:13Z
dc.date.issued2016-08-18
dc.identifier.citationMAN MOHAN SHRESTHA (2016-08-18). PHOSPHORYLATION OF TROPOMODULIN3 BY AMPK REGULATES GLUT4 TRANSLOCATION AND GLUCOSE UPTAKE IN L6 MYOBLASTS. ScholarBank@NUS Repository.
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/134549
dc.description.abstractInsulin and muscle contractions mediate translocation of glucose transporter 4 (GLUT4) to the plasma membrane for glucose uptake in skeletal muscles. Tropomodulin3 (Tmod3), an actin-capping protein that blocks the elongation and de-polymerization of actin filaments at the pointed end, is an Akt2 substrate and plays a vital role in GLUT4 fusion with the plasma membrane and glucose uptake under insulin-stimulated condition. It is well understood that insulin promotes GLUT4 translocation and glucose uptake in muscles and adipocytes by PI3K/Akt signaling pathway. Although it is believed that AMPK (5’ adenosine monophosphate-activated protein kinase) signaling regulates GLUT4 translocation during muscle contraction, the exact regulating mechanisms are not clearly elucidated. Bioinformatics and biochemical analysis identified Ser25 residue of Tmod3 as a potential site of phosphorylation. Ser25 phosphorylation was required for activated-AMPK-induced GLUT4 translocation and glucose uptake in L6 myoblasts, providing new mechanistic insights in the regulation of glucose homeostasis. Furthermore, Tmod3 played a key role in AMPK-induced actin re-modeling and in activated-AMPK-stimulated GLUT4 fusion with the plasma membrane and glucose uptake. Tmod3 is identified as a key downstream effector of AMPK in the regulation of GLUT4 translocation and glucose homeostasis in muscle cells.
dc.language.isoen
dc.subjectTmod3, AMPK, GLUT4, Glucose uptake, Actin Remodeling, Tropomyosin
dc.typeThesis
dc.contributor.departmentBIOCHEMISTRY
dc.contributor.supervisorHAN WEIPING
dc.description.degreePh.D
dc.description.degreeconferredDOCTOR OF PHILOSOPHY
dc.identifier.isiutNOT_IN_WOS
Appears in Collections:Ph.D Theses (Open)

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