Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/134391
Title: ENGINEERING THERAPEUTIC ANTIBODY CANDIDATES FOR DENGUE VIRUS
Authors: LIM SHE YAH
Keywords: Antibody, Dengue
Issue Date: 5-Aug-2016
Citation: LIM SHE YAH (2016-08-05). ENGINEERING THERAPEUTIC ANTIBODY CANDIDATES FOR DENGUE VIRUS. ScholarBank@NUS Repository.
Abstract: Dengue is currently the most important mosquito-borne viral disease affecting the population worldwide. Yet, there are no effective therapeutics available. Recently, our laboratory has characterized a potently neutralizing antibody against dengue virus serotype 1 (DENV-1) known as 14c10. For the comprehensive pharmacokinetics and off-target binding analysis of 14c10 as part of its clinical development with GlaxoSmithKline, an anti-idiotypic antibody highly specific for 14c10 was isolated. As part of our investigation, we proposed that 14c10-like antibodies might be produced in the natural immune response against DENV-1. We also showed the feasibility of engineering bispecific antibodies (monovalent function on each arm) against viruses, with serotype-specific antibodies against DENV-1 and DENV-2. In the pursuit of a rapid and efficient method of antibody discovery, we used a novel B cell activation approach coupled with next generation sequencing for the isolation of an anti-DENV-2 based on neutralization in less than two months.
URI: http://scholarbank.nus.edu.sg/handle/10635/134391
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