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Title: Thermosensitive nanomagnetic particles for bio-separation
Keywords: Thermosensitive magnetic particles; N-isopropylacrylamide; Adsorption; Desorption; Isoelectric point; Lower critical solution temperature.
Issue Date: 30-Aug-2007
Citation: NABILA SHAMIM (2007-08-30). Thermosensitive nanomagnetic particles for bio-separation. ScholarBank@NUS Repository.
Abstract: Thermosensitive polymer coated nanomagnetic adsorbents were synthesized by seed polymerization using surface modified nanomagnetic particles as the seed. The Fe3O4 nanomagnetic particles were prepared by chemical precipitation of Fe2+ and Fe3+ salts in the ratio of 1:2 under alkaline and inert condition. These stable particles were polymerized using N-isopropylacrylamide (NIPAM) as the main monomer, methylene-bis-acrylamide as the crosslinker and potassium per sulfate as the initiator. The thermosensitive adsorbents were characterized by using different methods. TEM showed that the particle remained discrete with a mean diameter of 12 nm. Adsorption and desorption behavior of Bovine Serum Albumin (BSA) and lysozyme (LYZ) on surface modified magnetic nano-particles covered with thermosensitive (PNIPAM) polymer was investigated as functions of temperature, pH and ionic strength. The results showed that adsorption was temperature sensitive and adsorption/desorption behavior mainly dependent on the properties of the particlesa?? surface. The effect of pH showed that the maximum amount of protein was adsorbed near the isoelectric point of the protein. Desorption was carried out using different desorption mediums and result showed adsorption temperature on subsequent desorption was significant. Evaluation of the desorbed proteins and enzymatic activity were also investigated.
Appears in Collections:Ph.D Theses (Open)

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