Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/134022
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dc.titleThe neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) induces changes in the heme spin state of microsomal cytochrome P-450
dc.contributor.authorShahi, G.S.
dc.contributor.authorMoochhala, S.M.
dc.contributor.authorDas, N.P.
dc.contributor.authorSunamoto, J.
dc.contributor.authorTajima, K.
dc.date.accessioned2016-12-20T08:42:37Z
dc.date.available2016-12-20T08:42:37Z
dc.date.issued1990
dc.identifier.citationShahi, G.S., Moochhala, S.M., Das, N.P., Sunamoto, J., Tajima, K. (1990). The neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) induces changes in the heme spin state of microsomal cytochrome P-450. Biochemistry International 22 (5) : 895-902. ScholarBank@NUS Repository.
dc.identifier.issn01585231
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/134022
dc.description.abstractIn vitro studies on the nature of interaction of the neurotoxin MPTP with hepatic microsomal cytochrome P-450 were carried out. Spectral perturbation studies showed nitrogenous ligand type binding between MPTP and cytochrome P-450 with a peak at 423 nm and a broad trough at 400 nm. Scatchard analysis of MPTP-cytochrome P-450 binding suggested that MPTP binds to at least 2 species of cytochrome P-450 - a high affinity binding species with an apparent spectral dissociation constant (K(s)) of 372 μM and a low affinity species with K(s) of 37.6 mM. EPR studies confirmed that MPTP is a type II substrate for the forms of cytochrome P-450 with which it interacts and causes a shift from the high spin state of cytochrome P-450 to the low spin state. MPTP is, thus, likely to be an effective inhibitor of cytochrome P-450.
dc.typeArticle
dc.contributor.departmentPHYSIOLOGY
dc.contributor.departmentPHARMACOLOGY
dc.contributor.departmentBIOCHEMISTRY
dc.description.sourcetitleBiochemistry International
dc.description.volume22
dc.description.issue5
dc.description.page895-902
dc.description.codenBIIND
dc.identifier.isiutNOT_IN_WOS
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