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Title: Validation of [13C]urea breath test for Helicobacter pylori using a simple gas chromatograph-mass selective detector
Authors: Lee, H.-S. 
Gwee, K.A. 
Teng, L.-Y.
Kang, J.-Y. 
Yeoh, K.-G. 
Wee, A. 
Chua, B.-C.
Keywords: [13C]urea breath test
Gas chromatograph-mass selective detector
Helicobactor pylori
Isotope ratio analysis
Issue Date: 1998
Citation: Lee, H.-S., Gwee, K.A., Teng, L.-Y., Kang, J.-Y., Yeoh, K.-G., Wee, A., Chua, B.-C. (1998). Validation of [13C]urea breath test for Helicobacter pylori using a simple gas chromatograph-mass selective detector. European Journal of Gastroenterology and Hepatology 10 (7) : 569-572. ScholarBank@NUS Repository.
Abstract: Objective. Isotope ratio mass spectrometry (IRMS) is the accepted method for accurately measuring the 13CO2:12CO2 ratio in the non-invasive and non-readioactive [13C]urea breath test (13C-UBT) for Helicobactor pylori. The IRMS instrument, an expensive and highly specialized analyser, is rarely available. The objective of this project was to modify and validate the use of a simple bench-top gas chromatograph-mass selective detector (GC-MSD) for 13C-UBT. Methods. Breath samples from 71 patients were taken at baseline and 30 min after ingestion of 100 mg [13C]urea. The breath samples were analysed using GC-MSD in the selected ion monitoring mode. The reference 13CO2:12CO2 ratio was from NBS19 obtained from the US National Institute of Standards and Technology. 13CO2:12CO2 ratios of the breath samples were determined. Excess delta per thousand (per mil, δ‰) of the 30 min sample over the baseline (Δδ‰) of ≤ 6Δδ‰ was considered H. pylori positive. Results from 13C-UBT and histology determined blind to each other were compared. Results. The coefficient of variation of the reference 13CO2:12CO2 ratio was 0.06%. Using histology as the 'gold standard', the sensitivity (97.9%) and specificity (95.8%) of the GC-MSD 13C-UBT were comparable to those of other methods of H. pylori diagnosis. Conclusion. A gas chromatograph coupled to a mass selective detector that is available in many analytical and biomedical laboratories can be used for the 13C-UBT. This method will increase the availability and reduce the cost of this non-invasive, non-radioactive diagnostic test.
Source Title: European Journal of Gastroenterology and Hepatology
ISSN: 0954691X
Appears in Collections:Staff Publications

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