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Title: Proteomic analysis of B cells from CD38 knockout mice
Keywords: CD38, B cells, 2-DE, MALTI-TOF MS, RT-PCR, MACS
Issue Date: 18-Sep-2007
Citation: ZHANG NENG (2007-09-18). Proteomic analysis of B cells from CD38 knockout mice. ScholarBank@NUS Repository.
Abstract: CD38 is a 42-45 kDa type-II transmembrane glycoprotein expressed in many tissues and cell types. CD38 is initially identified as an ectoenzyme capable of converting I?-NAD+ to cyclic ADP-ribose (cADPR), a potent modulator of Ca2+-induced Ca2+-release via ryanodine-sensitive Ca2+ channel. CD38 displays activity as a cell surface receptor in lymphocytes and triggers a variety of responses including: cell proliferation, apoptosis, protein tyrosine phosphorylation, intracellular calcium mobilization, etc. The present study was to investigate differential protein expression in CD38 deficient splenic B cells using proteomic approach. Mouse splenic B cells were isolated using magnetic-activated cell sorting (MACS) and its purity was ascertained by flow cytometry. Proteins of purified splenic B cells were subjected to two-dimensional gel electrophoresis (2-DE) and differentially expressed protein spots were analysed by MALDI-TOF mass spectrometry. The differential expression status of the candidate proteins were further analysed by RT-PCR. A total of 17 differentially expressed proteins were identified in the present studies and some of them are consistent with a role of CD38 in cell survival pathway and as an immunoregulatory molecule.
Appears in Collections:Master's Theses (Open)

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