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Title: Some characteristics of Δ1-pyrroline-5-carboxylate dehydrogenase in rat cerebellum
Authors: Wong, P.T.-H. 
Teo, W.L.
Leong, S.F. 
Issue Date: 1985
Citation: Wong, P.T.-H., Teo, W.L., Leong, S.F. (1985). Some characteristics of Δ1-pyrroline-5-carboxylate dehydrogenase in rat cerebellum. Neurochemistry International 7 (1) : 45-49. ScholarBank@NUS Repository.
Abstract: Δ1-Pyrroline-5-carboxylate dehydrogenase (P5CDH) in rat cerebellum was assayed with a simple spectrophotometric method using high-speed supernatants of whole tissue homogenates. Kinetic analysis showed that the enzyme has Km values of 0.83 ± 0.21 mM for Δ1-pyrroline-5-carboxylate and 0.47 ± 0.02 mM for NAD+. Various cations inhibited P5CDH but only at relatively high concentrations. Several amino acids were strongly inhibitory in the order GABA > glycine > hydroxyproline > cysteine {reversed tilde equals} proline > glutamine > glutamate > alanine. © 1985.
Source Title: Neurochemistry International
ISSN: 01970186
Appears in Collections:Staff Publications

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