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Title: | Role of interleukin-13 on the development of minimal change nephrotic syndrome - A novel animal model | Authors: | LAI KIN WAI | Keywords: | Interleukin-13, MCNS, nephrin, podocin, dystroglycan, B7-1 | Issue Date: | 29-Aug-2007 | Citation: | LAI KIN WAI (2007-08-29). Role of interleukin-13 on the development of minimal change nephrotic syndrome - A novel animal model. ScholarBank@NUS Repository. | Abstract: | IL-13 has been implicated in the pathogenesis of minimal change nephrotic syndrome (MCNS). This study aimed to investigate the role of IL-13 on the development of proteinuria and expression of podocyte-related genes associated with nephrotic syndrome. IL-13 was overexpressed in Wistar rats through transfection of a mammalian expression vector cloned with the rat IL-13 gene by in-vivo electroporation. The IL-13 transfected rats (n=41) showed significant proteinuria, hypoalbuminemia and hypercholesterolemia when compared to control rats (n=17). No significant histological changes were observed in the glomeruli of IL-13- transfected rats except effacement of podocyte foot processes. Glomerular gene and protein expression was significantly upregulated for B7-1 and IL-4RI?, but downregulated for nephrin, podocin and dystroglycan in IL-13-transfected rats compared to controls. In conclusion, overexpression of IL-13 causes podocyte injury and induces minimal change-like nephropathy in rat. | URI: | http://scholarbank.nus.edu.sg/handle/10635/13397 |
Appears in Collections: | Ph.D Theses (Open) |
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Lai Kin Wai PhD Thesis (Final version).pdf | 4.69 MB | Adobe PDF | OPEN | None | View/Download |
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