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dc.titleApplication of HCRSV protein cage for anticancer drug delivery
dc.contributor.authorREN YUPENG
dc.identifier.citationREN YUPENG (2007-09-18). Application of HCRSV protein cage for anticancer drug delivery. ScholarBank@NUS Repository.
dc.description.abstractThe icosahedral protein cage (PC) of the Hibiscus chlorotic ringspot virus (HCRSV) was explored as a platform for anticancer drug delivery. PC was prepared by removing viral RNA from coat proteins by dialysis against a Ca2+-deficient buffer of pH 8.0, and reassembling the coat proteins by dialysis with a Ca2+-containing buffer of pH 5.0. Using polyacids and dextrans as exemplary cargoes, it was found that only polyacids with MW above 13 kDa were successfully loaded into the PC. A novel method, named a??polyacid associationa??, was developed to load doxorubicin in the PC, which was conjugated with surface folic acid for targeting to cancer cells. The resulting fPC-Dox system had comparable morphology and physical properties as the HCRSV. It showed higher in vitro cytotoxicity against OVCAR-3 and CNE-1 cancer cells than against the representative normal CCL-186 cells, suggesting that the HCRSV PC had potential for targeted anticancer drug delivery.
dc.subjectPlant virus, Hibiscus chlorotic ringspot virus, Protein cage, Anticancer drug, Doxorubicin, Polyacid association
dc.contributor.supervisorCHAN LAI WAH
dc.description.degreeconferredDOCTOR OF PHILOSOPHY
Appears in Collections:Ph.D Theses (Open)

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