Please use this identifier to cite or link to this item:
Title: Tumour suppressor p53 and Rb genes in human hepatocellular carcinoma
Authors: Lin, Y.
Shi, C.Y. 
Li, B. 
Soo, B.H.
Mohammed-Ali, S.
Wee, A. 
Oon, C.J.
Mack, P.O.P.
Chan, S.H. 
Keywords: Aflatoxin
Hepatitis B vrius
Restriction fragment length polymorphism
Issue Date: Jan-1996
Citation: Lin, Y., Shi, C.Y., Li, B., Soo, B.H., Mohammed-Ali, S., Wee, A., Oon, C.J., Mack, P.O.P., Chan, S.H. (1996-01). Tumour suppressor p53 and Rb genes in human hepatocellular carcinoma. Annals of the Academy of Medicine Singapore 25 (1) : 22-30. ScholarBank@NUS Repository.
Abstract: Aberrations of the p53 and Rb tumour suppressor genes were examined in 12 human hepatocellular carcinoma (HCC)-derived cell lines from different geographic areas and 9 local HCCs by restriction fragment length polymorphisms (RFLP), polymerase chain reaction-single-strand conformation polymorphisms (PCR-SSCP) and DNA sequencing. The relationships between genetic changes and hepatitis B virus (HBV) DNA integration in samples were compared. None of the cell lines and tumours showed structural changes in the Rb gene, while 6 cell lines and 2 tumours had mutation or deletion in exons 5 to 8 of p53. Mutations include an AGG→AGT (Arg→Ser) transversion at codon 249 in PLC/PRF/5 and Mahlavu, an AAT→AAA (Asn→Cys) transversion at codon 200 in TONG/HCC, an AAG→GAG (Lys→Glu) transition at codon 139 in HCC-T, a CAT→CGT (His→Arg) transition at codon 214 in SC4, and a CCC→CTC (Pro→Leu) transition at codon 250 in SC8. In Huh4, an 18-bp deletion from codon 264 to 270 resulted in loss of Leu-Gly-Arg-Asn-Ser-Phe from the amino acid sequences 265 to 270, whereas Hep3B had a 7-kb deletion after exon 7 of p53. Our data indicate that whereas Rb may not have pleiotropic effects on HCC, p53 aberrations are frequently involved in hepatocarcinogenesis. Further, HBV infection appears to be unrelated to the micro-genetic changes of p53. The G to T codon-249-mutation is consistent with HCCs arising from areas at high risk for both aflatoxin B1 (AFB1) exposure and HBV infection.
Source Title: Annals of the Academy of Medicine Singapore
ISSN: 03044602
Appears in Collections:Staff Publications

Show full item record
Files in This Item:
There are no files associated with this item.

Page view(s)

checked on Jun 20, 2019

Google ScholarTM


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.