Please use this identifier to cite or link to this item:
|Title:||In vitro regeneration of Acacia mangium via organogenesis||Authors:||Xie, D.
|Issue Date:||2001||Citation:||Xie, D., Hong, Y. (2001). In vitro regeneration of Acacia mangium via organogenesis. Plant Cell, Tissue and Organ Culture 66 (3) : 167-173. ScholarBank@NUS Repository. https://doi.org/10.1023/A:1010632619342||Abstract:||Plant regeneration of Acacia mangium was achieved through organogenesis in callus cultures. Calli were induced from five types of explants (embryo axes and cotyledons of mature zygotic embryos as well as leaflets, petioles and stems of seedlings) of A. mangium on MS (Murashige and Skoog, 1962) basal medium containing 9.05 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 13.95 μM kinetin (KT). Green or green purple compact nodules containing clusters of meristematic centers were induced in these calli after transfer to MS basal medium containing 1.14-22.75 μM thidiazuron (TDZ) and 1.43-2.86 μM indole-3-acetic acid (IAA). A combination of 4.55 μM TDZ and 1.43 μM IAA promoted the highest percentage of calli to form nodules, in 8-11% of calli derived from cotyledons, embryo axes, leaflets or petiole and in 4% of calli derived from stems. Twenty-two percent of the nodules formed adventitious shoots on MS basal medium containing 0.045 μM TDZ. Shoots were elongated on MS medium containing 0.045 μM TDZ supplemented with 7.22 μM gibberellic acid. The medium containing 10.75 μM NAA and 2.33 μM KT promoted rooting of 10% of the elongated shoots. Plantlets grew up well in the green house.||Source Title:||Plant Cell, Tissue and Organ Culture||URI:||http://scholarbank.nus.edu.sg/handle/10635/133340||ISSN:||01676857||DOI:||10.1023/A:1010632619342|
|Appears in Collections:||Staff Publications|
Show full item record
Files in This Item:
There are no files associated with this item.
checked on Dec 3, 2019
WEB OF SCIENCETM
checked on Nov 26, 2019
checked on Nov 29, 2019
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.