Please use this identifier to cite or link to this item:
https://scholarbank.nus.edu.sg/handle/10635/13290
DC Field | Value | |
---|---|---|
dc.title | Anti-cancer mechanisms of indirubin-3'-monoxime | |
dc.contributor.author | SHI JIE | |
dc.date.accessioned | 2010-04-08T10:31:41Z | |
dc.date.available | 2010-04-08T10:31:41Z | |
dc.date.issued | 2008-04-28 | |
dc.identifier.citation | SHI JIE (2008-04-28). Anti-cancer mechanisms of indirubin-3'-monoxime. ScholarBank@NUS Repository. | |
dc.identifier.uri | http://scholarbank.nus.edu.sg/handle/10635/13290 | |
dc.description.abstract | Indirubin-3b -monoxime (I3M) is a derivative of indirubin, an active component from a Chinese medicinal recipe with known anti-cancer function. In this study, we focused on the molecular mechanism underlying I3M-induced apoptosis, especially the critical role of the pro-apoptosis Bcl-2 family members. We first observed I3M-induced apoptosis in a time- and dose-dependent manner in three different types of human cancer cells. Induction of the caspase cascade for both the extrinsic and intrinsic pathways was demonstrated. Initiation of the death receptor pathway started with enhanced surface expression and protein level of DR4 and DR5, which correlated with the up-regulation of p53 and its transcriptional activity. Importantly, in HeLa cells caspase-8 activation resulted in Bid cleavage, followed by Bax conformational change. Consistently, stable knockdown of Bid abrogated I3M-induced Bax conformational change and cell death. Moreover, ectopic expression of a viral caspase inhibitor or Bcl-2 partially protected I3M-induced apoptosis. | |
dc.language.iso | en | |
dc.subject | indirubin-3'-monoxime; death receptor; Bid; Bax; Apoptosis | |
dc.type | Thesis | |
dc.contributor.department | COMMUNITY,OCCUPATIONAL & FAMILY MEDICINE | |
dc.contributor.supervisor | SHEN HAN MING | |
dc.description.degree | Master's | |
dc.description.degreeconferred | MASTER OF SCIENCE | |
dc.identifier.isiut | NOT_IN_WOS | |
Appears in Collections: | Master's Theses (Open) |
Show simple item record
Files in This Item:
File | Description | Size | Format | Access Settings | Version | |
---|---|---|---|---|---|---|
ShiJ.pdf | 2.6 MB | Adobe PDF | OPEN | None | View/Download |
Google ScholarTM
Check
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.