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https://doi.org/10.1016/S0378-1135(99)00190-X
Title: | Overexpression and immunogenicity of the Oma87 outer membrane protein of Pasteurella multocida | Authors: | Mitchison, M. Wei, L. Kwang, J. Wilkie, I. Adler, B. |
Keywords: | Immunization Oma87 Pasteurella multocida |
Issue Date: | 1-Mar-2000 | Citation: | Mitchison, M., Wei, L., Kwang, J., Wilkie, I., Adler, B. (2000-03-01). Overexpression and immunogenicity of the Oma87 outer membrane protein of Pasteurella multocida. Veterinary Microbiology 72 (1-2) : 91-96. ScholarBank@NUS Repository. https://doi.org/10.1016/S0378-1135(99)00190-X | Abstract: | The outer membrane protein of Oma87 from Pasteurella multocida A:1 has significant similarity to the D15 protective antigen of Haemophilus influenzae (Ruffolo and Adler, 1996). Four fragments of Oma87 from a P. multocida serotype D strain were cloned into a pGEX expression vector and transformed into E. coli JM105. Western blot analysis revealed that convalescent chicken sera reacted with only GST-F1 fusion protein which contained amino acids 18 through to 130 of Oma87 fused to the GST protein. Vaccination with the GST-F1 protein failed to protect chickens against challenge with a virulent P. multocida serotype A. Copyright (C) 2000 Elsevier Science B.V. | Source Title: | Veterinary Microbiology | URI: | http://scholarbank.nus.edu.sg/handle/10635/132891 | ISSN: | 03781135 | DOI: | 10.1016/S0378-1135(99)00190-X |
Appears in Collections: | Staff Publications |
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