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|Title:||Processing scarce biological samples for light and transmission electron microscopy||Authors:||Taupin, P.||Keywords:||Araldite
Bovine serum albumin
|Issue Date:||Apr-2008||Citation:||Taupin, P. (2008-04). Processing scarce biological samples for light and transmission electron microscopy. European Journal of Histochemistry 52 (2) : 135-139. ScholarBank@NUS Repository.||Abstract:||Light microscopy (LM) and transmission electron microscopy (TEM) aim at understanding the relationship structure-function. With advances in biology, isolation and purification of scarce populations of cells or subcellular structures may not lead to enough biological material, for processing for LM and TEM. A protocol for preparation of scarce biological samples is presented. It is based on pre-embedding the biological samples, suspensions or pellets, in bovine serum albumin (BSA) and bis-acrylamide (BA), cross-linked and polymerized. This preparation provides a simple and reproducible technique to process biological materials, present in limited quantities that can not be amplified, for light and transmission electron microscopy. ©2008 European Journal of Histochemistry.||Source Title:||European Journal of Histochemistry||URI:||http://scholarbank.nus.edu.sg/handle/10635/132813||ISSN:||1121760X|
|Appears in Collections:||Staff Publications|
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