Please use this identifier to cite or link to this item: https://doi.org/10.2741/2021
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dc.titleProfiling of hepatocellular proteins by 1D PAGE-MALDI/MS/MS in a rat heat stress model
dc.contributor.authorRajaseger, G.
dc.contributor.authorLim, C.L.
dc.contributor.authorLee, K.W.
dc.contributor.authorArjunan, P.
dc.contributor.authorJia, L.
dc.contributor.authorMoochhala, S.
dc.date.accessioned2016-12-13T05:36:32Z
dc.date.available2016-12-13T05:36:32Z
dc.date.issued2006
dc.identifier.citationRajaseger, G., Lim, C.L., Lee, K.W., Arjunan, P., Jia, L., Moochhala, S. (2006). Profiling of hepatocellular proteins by 1D PAGE-MALDI/MS/MS in a rat heat stress model. Frontiers in Bioscience 11 (SUPPL. 3) : 2924-2928. ScholarBank@NUS Repository. https://doi.org/10.2741/2021
dc.identifier.issn10939946
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/132787
dc.description.abstractHeat induced complications cause an increase in a large number of proteins which play a role in diverse pathways during heat shock. A detailed characterization of these proteins is essential for understanding the molecular mechanisms involved in heat stroke. In this report, the proteins present in rat liver were compared at 37 °C (control) and at core temperature (Tc) 42 °C (heat stress) by 1D PAGE and MALDI/MS/MS. Among proteins identified in the sample after heat stress are dimethyglycine dehydrogenase, transketolase, carboxylic ester hydrolase, pyruvate kinase, L-type pyruvate kinase, arginosuccinate synthetase; fumarylacetoacetate hydrolase and peptidylpropyl isomerase A. These findings show that analysis of large scale proteins by MALDI/MS/MS provides a better understanding of the molecular mechanisms associated with heat shock. The resolution of proteins examined by 1D-PAGE was less than that obtained with 2D-PAGE. More specifically, 2D-PAGE allows better identification of low molecular weight proteins that can not be resolved by 1D-PAGE.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.2741/2021
dc.sourceScopus
dc.subject1D PAGE
dc.subjectHeat stress
dc.subjectHepatocellular proteins
dc.subjectMALDI
dc.subjectMS
dc.subjectPolyacrylamide gel electrophoresis
dc.subjectRat model
dc.typeArticle
dc.contributor.departmentANATOMY
dc.description.doi10.2741/2021
dc.description.sourcetitleFrontiers in Bioscience
dc.description.volume11
dc.description.issueSUPPL. 3
dc.description.page2924-2928
dc.identifier.isiut000237382800080
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