Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/132576
Title: Ongoing normal pregnancy after transfer of zona-free blastocysts: Implications for embryo transfer in the human
Authors: Fong, C.-Y. 
Bongso, A. 
Ng, S.C. 
Anandakumar, C. 
Trounson, A.
Ratnam, S. 
Keywords: Blastocyst transfer
Co-culture
IVF
Pregnancy
Zona-free blastocysts
Issue Date: Mar-1997
Citation: Fong, C.-Y., Bongso, A., Ng, S.C., Anandakumar, C., Trounson, A., Ratnam, S. (1997-03). Ongoing normal pregnancy after transfer of zona-free blastocysts: Implications for embryo transfer in the human. Human Reproduction 12 (3) : 557-560. ScholarBank@NUS Repository.
Abstract: Recent reports suggest that transfer of day 5 blastocysts improves implantation rates in in-vitro fertilization programmes. This paper reports a successful ongoing pregnancy after the transfer of zona-free day 6 expanded and hatching blastocysts. The patient was 37 years old and had undergone six stimulated and two thaw cycles previously, all of which had failed. Stimulation was by down-regulation and oocytes recovered transvaginally by ultrasound guidance. Two pronuclear embryos were co-cultured on Vero cells to day 6. The zonae of two hatching and two fully expanded blastocysts were removed using 0.5% pronase, and the zona-free blastocysts were then transferred. Pregnancy was confirmed on day 18 with a positive human chorionic gonadotrophin (HCG) and ultrasound at 6 weeks showed a single healthy fetal heart inside a clear sac. At 14 weeks a triple test (oestriol, J-HCG and α-fetoprotein) was normal and at 22 weeks a detailed ultrasound scan showed no congenital anomalies. This is the first report in the human of a normal ongoing pregnancy after the transfer of zona-free day 6 embryos.
Source Title: Human Reproduction
URI: http://scholarbank.nus.edu.sg/handle/10635/132576
ISSN: 02681161
Appears in Collections:Staff Publications

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