Please use this identifier to cite or link to this item: https://doi.org/10.1128/JVI.78.19.10543-10555.2004
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dc.titleInfectious entry of West Nile virus occurs through a clathrin-mediated endocytic pathway
dc.contributor.authorChu, J.J.H.
dc.contributor.authorNg, M.L.
dc.date.accessioned2016-12-13T05:33:42Z
dc.date.available2016-12-13T05:33:42Z
dc.date.issued2004-10
dc.identifier.citationChu, J.J.H., Ng, M.L. (2004-10). Infectious entry of West Nile virus occurs through a clathrin-mediated endocytic pathway. Journal of Virology 78 (19) : 10543-10555. ScholarBank@NUS Repository. https://doi.org/10.1128/JVI.78.19.10543-10555.2004
dc.identifier.issn0022538X
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/132546
dc.description.abstractThe pathway of West Nile flavivirus early internalization events was mapped in detail in this study. Overexpression of dominant-negative mutants of Eps15 strongly inhibits West Nile virus (WNV) internalization, and pharmacological drugs that blocks clathrin also caused a marked reduction in virus entry but not caveola-dependent endocytosis inhibitory agent, filipin. Using immunocryoelectron microscopy, WNV particles were seen within clathrin-coated pits after 2 min postinfection. Double-labeling immunofluorescence assays and immunoelectron microscopy performed with anti-WNV envelope or capsid proteins and cellular markers (EEA1 and LAMP1) revealed the trafficking pathway of internalized virus particles from early endosomes to lysosomes and finally the uncoating of the virus particles. Disruption of host cell cytoskeleton (actin filaments and microtubules) with cytochalasin D and nocodazole showed significant reduction in virus infectivity. Actin filaments are shown to be essential during the initial penetration of the virus across the plasma membrane, whereas microtubules are involved in the trafficking of internalized virus from early endosomes to lysosomes for uncoating. Cells treated with lysosomotropic agents were largely resistant to infection, indicating that a low-pH-dependent step is required for WNV infection. In situ hybridization of DNA probes specific for viral RNA demonstrated the trafficking of uncoated viral RNA genomes to the endoplasmic reticulum.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1128/JVI.78.19.10543-10555.2004
dc.sourceScopus
dc.typeArticle
dc.contributor.departmentMICROBIOLOGY
dc.description.doi10.1128/JVI.78.19.10543-10555.2004
dc.description.sourcetitleJournal of Virology
dc.description.volume78
dc.description.issue19
dc.description.page10543-10555
dc.description.codenJOVIA
dc.identifier.isiut000223964300036
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