Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/132270
Title: THE ROLE OF HFLX, A HIGHLY CONSERVED GTPASE, IN MYCOBACTERIUM TUBERCULOSIS
Authors: NG SZE WAI
Keywords: hflX, mycobacterium tuberculosis, GTPase, universal conservation
Issue Date: 27-Jun-2016
Citation: NG SZE WAI (2016-06-27). THE ROLE OF HFLX, A HIGHLY CONSERVED GTPASE, IN MYCOBACTERIUM TUBERCULOSIS. ScholarBank@NUS Repository.
Abstract: HflX is a universally conserved prokaryotic GTPase (ucpGTPase) which functions as a probable ribosomal-splitting factor in mycobacteria. Consistently, the absence of HflX was found to have a substantial amount of impact on BCG survivability under in vitro hypoxic conditions but not in a nutrient-starved environment. However, hflX deletion failed to impair BCG viability during infection of primary macrophages, and in the mouse lung and spleen. Instead, the ΔhflX BCG strain displayed a growth advantage compared to its parental counterpart in both models, suggesting HflX being a negative regulator of mycobacterial growth rate. HflX structure was observed to be significantly conserved in our computational analyses and we subsequently confirmed its role in preserving mycobacterial viability in BCG when exposed to elevated temperatures. In conclusion, HflX characterisation is the first step in understanding the role of conserved GTPases in pathogenic mycobacteria, especially in mycobacterial growth and survival.
URI: http://scholarbank.nus.edu.sg/handle/10635/132270
Appears in Collections:Master's Theses (Open)

Show full item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
NgSW.pdf2.27 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

31
checked on Feb 1, 2019

Download(s)

1
checked on Feb 1, 2019

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.