Please use this identifier to cite or link to this item: https://scholarbank.nus.edu.sg/handle/10635/132169
DC FieldValue
dc.titleDEVELOPMENT AND APPLICATION OF A NEGATIVE SELECTION SYSTEM FOR STUDYING THE GENETIC BASIS OF VIRULENCE IN ISOLATES OF ENTEROBACTERIACEAE
dc.contributor.authorKHETRAPAL VARNICA
dc.date.accessioned2016-12-05T18:00:12Z
dc.date.available2016-12-05T18:00:12Z
dc.date.issued2016-08-08
dc.identifier.citationKHETRAPAL VARNICA (2016-08-08). DEVELOPMENT AND APPLICATION OF A NEGATIVE SELECTION SYSTEM FOR STUDYING THE GENETIC BASIS OF VIRULENCE IN ISOLATES OF ENTEROBACTERIACEAE. ScholarBank@NUS Repository.
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/132169
dc.description.abstractWe have designed a general negative selection strategy based on inducible toxins. Combined with an appropriate positive selection marker, multiple variants of this strategy are efficient for making chromosomal alterations in both lab strains of E. coli and clinical isolates of uropathogenic and enterohemorrhagic E. coli, and Salmonella enterica. Importantly, no strain- or species-specific optimization is needed. Furthermore, our negative selection cassette demonstrates up to 60-fold greater selection stringency than all other reported negative selection systems and up to a 1000-fold greater than the most widely used negative selection system in E. coli. Taking advantage of the high stringency of our system, we have worked towards establishing mass allelic exchange between different strains of E. coli. By combining sexual genetics and linkage analysis, we hope to decipher causal genotype-phenotype associations in bacteria. We have also enabled an experimental method to identify regions of the bacterial genome that have variable stability.
dc.language.isoen
dc.subjectNegative selection, UPEC, genome engineering, allelic exchange, bacterial genome instability
dc.typeThesis
dc.contributor.departmentMEDICINE
dc.contributor.supervisorSWAINE LIN CHEN
dc.description.degreePh.D
dc.description.degreeconferredDOCTOR OF PHILOSOPHY
dc.identifier.isiutNOT_IN_WOS
Appears in Collections:Ph.D Theses (Open)

Show simple item record
Files in This Item:
File Description SizeFormatAccess SettingsVersion 
KhetrapalV.pdf21.34 MBAdobe PDF

OPEN

NoneView/Download

Page view(s)

71
checked on Feb 2, 2023

Download(s)

15
checked on Feb 2, 2023

Google ScholarTM

Check


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.