Please use this identifier to cite or link to this item: https://doi.org/10.1016/S0378-1097(99)00491-7
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dc.titleSequence analysis of a new open reading frame located in the pathogenicity locus of Clostridium difficile strain 8864
dc.contributor.authorSong, K.P.
dc.contributor.authorOw, S.E.
dc.contributor.authorChang, S.Y.
dc.contributor.authorBai, X.L.
dc.date.accessioned2016-11-29T02:51:12Z
dc.date.available2016-11-29T02:51:12Z
dc.date.issued1999-11-15
dc.identifier.citationSong, K.P., Ow, S.E., Chang, S.Y., Bai, X.L. (1999-11-15). Sequence analysis of a new open reading frame located in the pathogenicity locus of Clostridium difficile strain 8864. FEMS Microbiology Letters 180 (2) : 241-248. ScholarBank@NUS Repository. https://doi.org/10.1016/S0378-1097(99)00491-7
dc.identifier.issn03781097
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/132009
dc.description.abstractStrain 8864 is a natural isolate of Clostridium difficile that is toxin B-positive and toxin A-negative. Recent work showed that there is a genetic rearrangement occurring at the pathogenicity locus (PaLoc) of the bacteria. Our investigation in the PaLoc region revealed an open reading frame (tcdF) of 543 bp DNA not reported before. This tcdF could encode a putative polypeptide of 22 kDa. Although no peptide homology was found with other known proteins, we postulate that it could be a novel protein because not only highly consensus ribosome-binding and promoter sequences were found upstream of tcdF, transcript was also identified at the region occupied by tcdF. Copyright (C) 1999 Federation of European Microbiological Societies.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1016/S0378-1097(99)00491-7
dc.sourceScopus
dc.subjectRecombinant DNA
dc.subjectSequence analysis
dc.subjectToxinogenic Clostridium difficile
dc.typeArticle
dc.contributor.departmentMICROBIOLOGY
dc.description.doi10.1016/S0378-1097(99)00491-7
dc.description.sourcetitleFEMS Microbiology Letters
dc.description.volume180
dc.description.issue2
dc.description.page241-248
dc.description.codenFMLED
dc.identifier.isiut000083659000017
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