Please use this identifier to cite or link to this item: https://doi.org/10.1080/00313029600169114
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dc.titleRapid genotypic confirmation of methicillin resistance
dc.contributor.authorInglis, T.J.J.
dc.contributor.authorRahman, W.
dc.date.accessioned2016-11-29T02:51:10Z
dc.date.available2016-11-29T02:51:10Z
dc.date.issued1996
dc.identifier.citationInglis, T.J.J., Rahman, W. (1996). Rapid genotypic confirmation of methicillin resistance. Pathology 28 (3) : 259-261. ScholarBank@NUS Repository. https://doi.org/10.1080/00313029600169114
dc.identifier.issn00313025
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/132007
dc.description.abstractDetection of phenotypic methicillin resistance in Staphylococcus aureus clinical strains by conventional disk diffusion testing is fraught with problems. We used gene amplification of the mecA locus by polymerase chain reaction (PCR), in conjunction with a capillary/air thermal cycler, to overcome both the inaccuracy of phenotypic methods and the lengthy processing times required for previous genotypic methods. The rapid PCR method correctly identified methicillin resistance in a consecutive series of 30 S. aureus isolates when compared with routine and reference phenotypic methods. The shorter processing time and smaller reagent volumes required for the air thermal cycler make same-day determination of methicillin resistance in clinical isolates feasible for diagnostic laboratories.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1080/00313029600169114
dc.sourceScopus
dc.subjectCapillary/air thermal cycler
dc.subjectMethicillin resistant Staphylococcus aureus
dc.subjectPCR
dc.typeArticle
dc.contributor.departmentMICROBIOLOGY
dc.description.doi10.1080/00313029600169114
dc.description.sourcetitlePathology
dc.description.volume28
dc.description.issue3
dc.description.page259-261
dc.description.codenPTLGA
dc.identifier.isiutA1996VY43600012
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