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Title: The Effects of the histones and histone-interacting partners on transcriptional regulation
Keywords: histone-interacting protein, protein-protein interaction, library screening, transcriptional regulation, histone modification, split-ubiquitin system
Issue Date: 24-May-2008
Citation: HE HONGPENG (2008-05-24). The Effects of the histones and histone-interacting partners on transcriptional regulation. ScholarBank@NUS Repository.
Abstract: In this project, yeast genomic DNA library was screened for histone-interacting proteins. Thirty-four proteins, including Paf1p, Rpb4p, Slm1p and Ynl010wp, were isolated as novel interactors for the histones H1, H2B, H3 and H4. Results of phenotype examination, quantitative real-time PCR assay and ChIP assay suggested that Paf1p, Rpb4p and Ynl010wp were recruited to HIS3 gene under activated condition where they were required for HIS3 activation, whereas Slm1p was found at the HIS3 gene, where it was required for HIS3 activation, regardless if the gene was activated or not. An examination of the modification status of H2B suggested that the transcriptional functions of Paf1p, Rpb4p, Slm1p and Ynl010wp on the HIS3 gene were correlated with their influence on the mono-ubiquitination status of histone H2B. The physical interactions between Paf1p, Rpb4p, Slm1p and Ynl010wp and H2B might help their association with chromatin where they facilitate the activated transcription and influence histone modification.
Appears in Collections:Ph.D Theses (Open)

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