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Title: Development of molecular diagnostics and antiviral therapy against enterovirus 71 (EV71)
Authors: TAN ENG LEE
Keywords: Enterovirus 71 (EV71), HFMD, rapid detection, real-time PCR, RNA interference (RNAi), potential antiviral strategy
Issue Date: 17-Apr-2008
Citation: TAN ENG LEE (2008-04-17). Development of molecular diagnostics and antiviral therapy against enterovirus 71 (EV71). ScholarBank@NUS Repository.
Abstract: Enterovirus 71 (EV71) and Coxsackievirus A16 (CA16) are main causative agents of Hand, Foot and Mouth Disease (HFMD) in young children. However, EV71 is associated with serious neurological complications and caused high mortalities in outbreaks in Asia Pacific. Currently, there is no rapid diagnostics to detect or effective antivirals to treat EV71. Here, one-step real-time PCR assays based on different chemistries to detect and differentiate EV71 from CA16 were developed. RNA interference (RNAi) was evaluated as potential antiviral strategy against EV71. Enhanced silencing of EV71 using synthetic 29-mer shRNAs was demonstrated in vitro. In evaluating different forms of siRNAs in vivo, both synthetic 19-mer siRNAs and plasmid-derived shRNAs were shown to inhibit EV71 infections in suckling mice. However, the synthetic 29-mer shRNAs did not protect the suckling mice from fatal EV71 infections despite being more potent in vitro. The results showed that RNAi is potentially a therapeutic strategy against EV71.
Appears in Collections:Ph.D Theses (Open)

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