Proteomics Analysis of Pseudomonas putida in Biodegradation of Aromatic Compounds

Abstract

In this research, proteomics analysis was used to study three previously reported phenomena in biodegradation involving Pseudomonas putida. In the first system, 36 differentially expressed proteins, including 8 catabolic enzymes, were identified. 49 proteins were identified in P. putida P8 grown on a cometabolic system containing 200 mg/L of phenol, 200 mg/L of 4-chlorophenol and 1000 mg/L sodium glutamate. Among them, 16 were found differentially expressed in the two exponential growth phases during biphasic growth. In the final system, 25 protein spots were identified in P. putida ATCC 17484 during growth in a cometabolic biodegradation system of carbazole (0.5 mg/L), sodium salicylate (200 mg/L) and p-cresol (10 mg/L or 70 mg/L). There were significant differences in the abundances of 8 proteins during growth at two typical p-cresol concentrations. Collectively, these results enhanced our understanding of the catabolic pathways and the physiological status of P. putida during biodegradation of aromatic compounds.