Please use this identifier to cite or link to this item: https://doi.org/10.1117/12.808524
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dc.titleImaging dental sections with polarization-resolved SHG and timeresolved autofluorescence
dc.contributor.authorChen, J.H.
dc.contributor.authorLin, P.-Y.
dc.contributor.authorHsu, S.C.Y.
dc.contributor.authorKao, F.-J.
dc.date.accessioned2016-11-29T01:20:37Z
dc.date.available2016-11-29T01:20:37Z
dc.date.issued2009
dc.identifier.citationChen, J.H., Lin, P.-Y., Hsu, S.C.Y., Kao, F.-J. (2009). Imaging dental sections with polarization-resolved SHG and timeresolved autofluorescence. Progress in Biomedical Optics and Imaging - Proceedings of SPIE 7183 : -. ScholarBank@NUS Repository. https://doi.org/10.1117/12.808524
dc.identifier.issn16057422
dc.identifier.urihttp://scholarbank.nus.edu.sg/handle/10635/131603
dc.description.abstractIn this study, we are using two-photon (2-p) excited autofluorescence and second harmonic (SH) as imaging modalities to investigate dental sections that contains the enamel and the dentin. The use of near-infrared wavelengths for multiphoton excitation greatly facilitates the observation of these sections due to the hard tissue's larger index of refraction and highly scattering nature. Clear imaging can be achieved without feature altering preparation procedures of the samples. Specifically, we perform polarization resolving on SH and lifetime analysis on autofluorescence. Polarization resolved SH reflects the preferred orientation of collagen while very different autofluorescence lifetimes are observed from the dentin and the enamel. The origin of 2-p autofluorescence and SH signals are attributed to hydroxyapatite crystals and collagen fibrils, respectively. Hydroxyapatite is found to be present throughout the sections while collagen fibrils exist only in the dentin and dentinoenamel junctions. © 2009 SPIE.
dc.description.urihttp://libproxy1.nus.edu.sg/login?url=http://dx.doi.org/10.1117/12.808524
dc.sourceScopus
dc.subjectAnisotropy
dc.subjectAutofluorescence
dc.subjectFluorescence lifetime imaging microscopy
dc.subjectSecond harmonic generation
dc.subjectTwo-photon excitation
dc.typeConference Paper
dc.contributor.departmentPREVENTIVE DENTISTRY
dc.description.doi10.1117/12.808524
dc.description.sourcetitleProgress in Biomedical Optics and Imaging - Proceedings of SPIE
dc.description.volume7183
dc.description.page-
dc.description.codenPSISD
dc.identifier.isiut000284822400038
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