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Title: Imaging dental sections with polarization-resolved SHG and timeresolved autofluorescence
Authors: Chen, J.H.
Lin, P.-Y.
Hsu, S.C.Y. 
Kao, F.-J.
Keywords: Anisotropy
Fluorescence lifetime imaging microscopy
Second harmonic generation
Two-photon excitation
Issue Date: 2009
Citation: Chen, J.H., Lin, P.-Y., Hsu, S.C.Y., Kao, F.-J. (2009). Imaging dental sections with polarization-resolved SHG and timeresolved autofluorescence. Progress in Biomedical Optics and Imaging - Proceedings of SPIE 7183 : -. ScholarBank@NUS Repository.
Abstract: In this study, we are using two-photon (2-p) excited autofluorescence and second harmonic (SH) as imaging modalities to investigate dental sections that contains the enamel and the dentin. The use of near-infrared wavelengths for multiphoton excitation greatly facilitates the observation of these sections due to the hard tissue's larger index of refraction and highly scattering nature. Clear imaging can be achieved without feature altering preparation procedures of the samples. Specifically, we perform polarization resolving on SH and lifetime analysis on autofluorescence. Polarization resolved SH reflects the preferred orientation of collagen while very different autofluorescence lifetimes are observed from the dentin and the enamel. The origin of 2-p autofluorescence and SH signals are attributed to hydroxyapatite crystals and collagen fibrils, respectively. Hydroxyapatite is found to be present throughout the sections while collagen fibrils exist only in the dentin and dentinoenamel junctions. © 2009 SPIE.
Source Title: Progress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN: 16057422
DOI: 10.1117/12.808524
Appears in Collections:Staff Publications

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